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SIMA: sequential image analysis

Date:September 08, 2016


SIMA (Sequential IMage Analysis) is an Open Source package for analysis of time-series imaging data arising from fluorescence microscopy. The functionality of this package includes:

  • correction of motion artifacts
  • segmentation of imaging fields into regions of interest (ROIs)
  • extraction of dynamic signals from ROIs

The included ROI Buddy software provides a graphical user interface (GUI) supporting the following functionality:

  • manual creation of ROIs
  • editing of ROIs resulting from automated segmentation
  • registration of ROIs across separate imaging sessions

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